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Bacterial identification of acne vulgaris

  • Lovena Sari ,
  • Nelva Karmila Jusuf ,
  • Imam Budi Putra ,


Background: Acne vulgaris (AV) is a chronic inflammation of the pilosebaceous unit with clinical polymorphic lesion consist of non-inflammatory (open and closed comedones) and inflammatory lesions (papules, pustules, and nodules) with varying degree of inflammation and depth. Earlier studies showed that other bacteria might also found and played a role in acne pathogenesis besides Cutibacterium acnes (C. acnes).

Patients and Methods:  This descriptive observational study used cross-sectional method. Samples were collected from 40 subjects with AV. We took the samples from non-inflammatory (closed comedones) and inflammatory lesions (pustule) in each subject, followed by Gram staining, aerobic and anaerobic bacterial culture, and bacterial identification. This research has been approved by the Ethical Committee, Faculty of Medicine, University of Sumatera Utara.

Results: There were 12 bacterial species that were identified from 80 samples. We identified Cutibacterium acnes (21,2%) in anaerobic culture. While in aerobic culture, we identified Staphylococcus epidermidis (47,5%), Staphylococcus hominis (17,5%), Staphylococcus aureus (8,7%), Staphylococcus haemolyticus (8,7%), Leuconostoc mesentroides (6,2%), Micrococcus luteus (3,7%), Kocuria varians (2,5%), Staphylococcus vitulinus (1,2%), Staphylococcus cohnii (1,2%), Staphylococcus arlettae (1,2%) and Dermacoccus nishinomyaensis (1,2%).

Conclusion: The two most common bacteria in acne vulgaris are Staphylococcus epidermidis and Cutibacterium acnes


  1. Goh C, Cheng C, Agak G, Zaenglein AL, Graber EM, Thiboutot Dm, Kim J. Acneiform Disorder. In: Kang S, Amagai M, Bruckner AL, Enk AH, Margolis DJ, McMichael AJ, Orringer JS, editors. Fitzpatrick’s dermatology in general medicine. First Volume. 9th editions. New York: The McGraw Hill Companies; 2019:1391-1418.
  2. Layton AM, Eady EA, Zouboulis CC. Acne. In: Griffith C, Barker J, Bleiker T, Chalmers R, Creamer D, editors. Rooks Textbook of Dermatology. Third Volume. 9th editions. United Kingdom: Wiley Blackwell; 2016: 90.1-67
  3. Barratt H, Hamilton F, Car J, Lyons C, Layton A, Majeed A. Outcomes measures in acne vulgaris: systematic review. British Journal of Dermatology. 2009;160(3):132-6.
  4. Kraft J, Freiman A. Management of acne. Can Med Assoc J. 2011; 183(7):430-5.
  5. Thiboutot D, Gollnick H, Bettoli V, DreÌno B, Kang S, Leyden JJ, et al. New insights into the management of acne: an update from the global alliance to improve outcomes in acne group. J Am Acad Dermatol. 2009;60(5 Suppl):1–50.
  6. Beylot C, Auffret N, Poli F, Claudel JP, Leccia MT, Del Giudice P, dkk. Propionibacterium acnes: An update on its role in the pathogenesis of acne. J Eur Acad Dermatol Venereol. 2014;28(3):271-8.
  7. Dreno B, Pecastaings S, Corver S, Veraldi S, Khammari A, Roques C. Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates. Journal of the European Academy of Dermatology and Venereology. 2018; 32(2):5-14
  8. Hassanzadeh P, Bahmani M, Mehrabani D. Bacterial resistance to antibiotics in acne vulgaris: An in vitro study. Indian J Dermatol. 2008;53(3):122.
  9. Rocha M, Bagatin E. Skin barrier and microbiome in acne. Arch Dermatol Res. 2017:1-5.
  10. Kumar B, Pathak R, Mary PB, Jha D, Sardana K, Gautam HK. New insights into acne pathogenesis: Exploring the role of acne-associated microbial populations. Derm Sinica. 2016;34(2):67-73.
  11. Skroza N, Tulino E, Mambrin A, Zuber S, Balduzzi V, et al. Adult Acne Versus Adolescent Acne: A Retrospective Study of 1.167 Patients. J Clin Aesthet Dermatol. 2018;11(1): 21-25.
  12. Eyuboglu M, Kalay I, Eyuboglu D. Evaluation of Adolescents Diagnosed with Acne Vulgaris for Qualityof Life and Psychosocial Challenges. Indian J Dermatol. 2018; 63(2): 131-35.
  13. Moon SH, Roh HS, Kim YH, Kim JE, Ko JY, Ro YS. Antibiotic resistance of microbial strains isolated from Korean acne patients. J Dermatol. 2012;39(10): 833-7.
  14. Sylvia L, Kusnandar E, Lestari S. Hubungan antara Jenis Mikroorganisme yang ditemukan pada Lesi Akne dengan Jenis Lesi Akne. [thesis]. Padang: Universitas Andalas; 2010.
  15. Srikanth M, Kalyani CS, Mohan N, Sridhar K, Padmaja IJ. Bacteriology of acne. Journal of Evolutions of Medical and Dental Sciences. 2015;4(19):3267-74.
  16. Syahrial MA, Nasution D, Jusuf NK, Lubis SE. Pola Resistensi Propionibacterium acnes Terhadap Antibiotika Oral Pada Pasien Akne Vulgaris di RSUP H. Adam Malik Medan. [thesis]. Medan: Universitas Sumatera Utara; 2009.
  17. Biswal I, Gaind R, Kumar N, Mohanty S, Manchanda V, Khunger N, dkk. In vitro antimicrobial susceptibility patterns of Propionibacterium acnes isolated from patients with acne vulgaris. J Infect Dev Ctries. 2016;10(10):1140-5.
  18. Süer K, Güvenir M. Propionibacterium acnes (Cutibacterium acnes) and acne vulgaris: The latest updates of antimicrobial activity. Turk J Dermatol. 2019;13:57-9.
  19. Leyden JJ. A review of the use of combination therapies for the treatment of acne vulgaris. J Am Acad Dermatol. 2003;49:S200-10.
  20. Zhang YQ, Ren SX, Li HL, Wang YX, Fu G et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol Microbiol. 2003;49(6):1577-93.
  21. Heilmann C, Schweitze O, Gerke C, Vanittanakom N, Mack D, Goetz F. Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol. 1996;20:1083-91.
  22. Vuong C, Voyich JM, Fischer ER, et al. Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system. Cell Microbiol. 2004;6:269-75.
  23. Fey PD, Olson ME. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol. 2010;5:917-33.

How to Cite

Sari, L., Jusuf, N. K., & Putra, I. B. (2020). Bacterial identification of acne vulgaris. Bali Medical Journal, 9(3), 753–756.




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