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The relationship of biofilm Staphylococcus aureus with degree of severity and infection in patients of chronic rhinosinusitis

  • Teuku Husni T.R ,
  • Karina Witary Ayu Siagian ,
  • Benny Kurnia ,

Abstract

Background: Staphylococcus aureus is the main pathogen infect chronic rhinosinusitis (CRS) and the biofilms play an essential role in the pathophysiology of infection and treatment of patients. However, research on clinical conditions is still limited. This study aimed to evaluate the relationship between S. aureus biofilms with severity and infection degree in CRS patients.

Methods: A post-test-only study was conducted at Dr Zaionel Abidin Hospital in Banda Aceh, Indonesia from January to April 2022. Thirty-five CRS patients, classified according to the disease severity and infection degree, were included and followed in the study. A mucosal swab on each patient was collected and biofilm formation analyzed quantitatively and qualitatively using a spectrophotometer and scanning electron microscope (SEM), respectively. The relationships between the bacterial growth, biofilm formation and mass of biofilms with the disease severity and degree of infection were assessed statistically.

Results: More than half of the patients (54.3%) were aged younger than 40 years, and 51.4% were male. Nasal congestion and rhinorrhoea were the most common reported obligate symptoms (78.2%) and 82.9% patients have additional symptom on facial pain. There was the relationship between bacterial growth and disease severity (p=0.046) and infection degree of CSR cases (p=0.031). Our data showed a significant association between biofilm formation and the disease severity (p=0.022) and infection degree of CSR patients (p=0.020). The mass of the biofilm was also associated significantly with disease severity (p=0.029) and degree of infection (p=0.018).

Conclusions: Our study shows that the formation and mass of biofilm associated with the disease severity and degree of infection in CSR. Biofilms may contribute to the clinical condition and also be considered during management of CRS patients.

References

  1. Brook I. Microbiology of chronic rhinosinusitis. European Journal of Clinical Microbiology & Infectious Diseases. 2016;35:1059-68.
  2. Hamilos DL. Chronic rhinosinusitis: epidemiology and medical management. J Allergy Clin Immunol. 2011;128:693-707.
  3. Lam K, Schleimer R, Kern RC. The Etiology and Pathogenesis of Chronic Rhinosinusitis: a Review of Current Hypotheses. Curr Allergy Asthma Rep. 2015;15:41-49.
  4. Al-Mutairi D, Kilty SJ. Bacterial biofilms and the pathophysiology of chronic rhinosinusitis. Curr Opin Allergy Clin Immunol. 2011;11:18-23.
  5. Shariati A, Vesal S, Khoshbayan A, Goudarzi P, Darban-Sarokhalil D, Razavi S, et al. Novel strategies for inhibition of bacterial biofilm in chronic rhinosinusitis. J Appl Microbiol. 2022; 132:2531-46.
  6. Foreman A, Jervis-Bardy J, Wormald PJ. Do biofilms contribute to the initiation and recalcitrance of chronic rhinosinusitis? Laryngoscope. 2011;121:1085-91.
  7. Ramadan HH, Sanclement JA, Thomas JG. Chronic rhinosinusitis and biofilms. Otolaryngol Head Neck Surg. 2005;132:414-7.
  8. Ceri H, Olson ME, Stremick C, Read RR, Morck D, Buret A. The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol. 1999;37:1771-6.
  9. Prince AA, Steiger JD, Khalid AN, Dogrhamji L, Reger C, Eau Claire S, et al. Prevalence of biofilm-forming bacteria in chronic rhinosinusitis. Am J Rhinol. 2008;22:239-45.
  10. Cirkovic I, Pavlovic B, Bozic DD, Jotic A, Bakic L, Milovanovic J. Antibiofilm effects of topical corticosteroids and intranasal saline in patients with chronic rhinosinusitis with nasal polyps depend on bacterial species and their biofilm-forming capacity. Eur Arch Otorhinolaryngol. 2017;274:1897-903.
  11. Fokkens WJ, Lund VJ, Hopkins C, Hellings PW, Kern R, Reitsma S, et al. European Position Paper on Rhinosinusitis and Nasal Polyps 2020. Rhinology. 2020;58:1-464.
  12. Lauber CL, Zhou N, Gordon JI, Knight R, Fierer N. Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples. FEMS Microbiol Lett. 2010;307:80-6.
  13. Becerra SC, Roy DC, Sanchez CJ, Christy RJ, Burmeister DM. An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue. BMC Res Notes. 2016;9:216.
  14. Jamal M, Ahmad W, Andleeb S, Jalil F, Imran M, Nawaz MA, et al. Bacterial biofilm and associated infections. J Chin Med Assoc. 2018;81:7-11.
  15. Sutton S. Accuracy of Plate Counts; 2011.
  16. Bose S, Grammer LC, Peters AT. Infectious Chronic Rhinosinusitis. J Allergy Clin Immunol Pract. 2016;4:584-9.
  17. Vickery TW, Ramakrishnan VR, Suh JD. The Role of Staphylococcus aureus in Patients with Chronic Sinusitis and Nasal Polyposis. Curr Allergy Asthma Rep. 2019;19:21-26.
  18. Boase S, Foreman A, Cleland E, Tan L, Melton-Kreft R, Pant H, et al. The microbiome of chronic rhinosinusitis: culture, molecular diagnostics and biofilm detection. BMC Infect Dis. 2013;13:210-218.
  19. Dlugaszewska J, Leszczynska M, Lenkowski M, Tatarska A, Pastusiak T, Szyfter W. The pathophysiological role of bacterial biofilms in chronic sinusitis. Eur Arch Otorhinolaryngol. 2016;273:1989-94.
  20. Sun Y, Zhou B, Wang CS, Huang Q, Zhang Q, Han YH, et al. Clinical and histopathologic features of biofilm-associated chronic rhinosinusitis with nasal polyps in Chinese patients. Chin Med J (Engl). 2012;125:1104-9.
  21. Silva MC, Werlang MH, Vandresen DF, Fortes PC, Pascotto CR, Lúcio LC, et al. Genetic, antimicrobial resistance profile and mortality rates of Acinetobacter baumannii infection in Brazil: A systematic review. Narra J. 2022;2:e68-e72.
  22. Fastenberg JH, Hsueh WD, Mustafa A, Akbar NA, Abuzeid WM. Biofilms in chronic rhinosinusitis: Pathophysiology and therapeutic strategies. World J Otorhinolaryngol Head Neck Surg. 2016; 2:219-29.
  23. Li H, Wang D, Sun X, Hu L, Yu H, Wang J. Relationship between bacterial biofilm and clinical features of patients with chronic rhinosinusitis. Eur Arch Otorhinolaryngol. 2012; 269:155-63.
  24. Panchatcharam BS, Cooksley CM, Ramezanpour M, Vediappan RS, Bassiouni A, Wormald PJ, et al. Staphylococcus aureus biofilm exoproteins are cytotoxic to human nasal epithelial barrier in chronic rhinosinusitis. Int Forum Allergy Rhinol. 2020;10:871-83.
  25. Jung JH, Cha HE, Kang IG, Kim ST. Clinical characteristics of biofilms in patients with chronic rhinosinusitis: a prospective case-control study. Indian J Otolaryngol Head Neck Surg. 2015;67:1-6.
  26. Young D, Morton R, Bartley J. Therapeutic ultrasound as treatment for chronic rhinosinusitis: preliminary observations. J Laryngol Otol. 2010;124:495-9.

How to Cite

Husni T.R, T. ., Siagian, K. W. A., & Kurnia, B. . (2023). The relationship of biofilm Staphylococcus aureus with degree of severity and infection in patients of chronic rhinosinusitis. Bali Medical Journal, 12(1), 884–887. https://doi.org/10.15562/bmj.v12i1.4069

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Teuku Husni T.R
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Karina Witary Ayu Siagian
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Benny Kurnia
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