Skip to main content Skip to main navigation menu Skip to site footer

Clinical manifestations of allergic reactions and immunoglobulin E levels on allogeneic freeze-dried platelet-rich plasma (PRP) application

  • Bernadetta ,
  • Magda Rosalina Hutagalung ,
  • Lynda Hariani ,

Abstract

Link of Video Abstract: https://youtu.be/yS431sCL3VQ

Background: Several studies have reported that modern wound dressings offer better results. Allogeneic PRP products in the form of injections and gels are available in the market, which helps accelerate wound healing without triggering an immunological reaction. Meanwhile, this study was carried out to investigate the Allogeneic Freeze-Dried PRP form, which is more practical, efficient, and safer. This study aims to determine the effect of Allogeneic Freeze-Dried PRP on human split-thickness skin graft donor wounds on clinical manifestations of allergic reactions and levels of Immunoglobulin E (IgE).

Methods: A randomized Pre- and Post-test Control-Trial Group Design was conducted on patients with split-thickness skin graft donor wounds using Allogeneic Freeze-Dried PRP dressings at Dr. Soetomo General Hospital, Surabaya. Allergic manifestations were reviewed during the observation period of up to 4 days, and IgE levels were measured pre-test and 60 minutes post-application. A statistical comparison of results was carried out between the trial and control groups that used paraffin dressings.

Results: A total of 36 patients were used as subjects, with 19 (52.78%) in the trial group and 17 (47.22%) in the control group, and their parameters were generally homogeneous. There were no clinical manifestations of allergic, changes in vital signs, and significant differences in IgE levels before and after application statistically in all study subjects (p>0.05).

Conclusion: Application of Allogeneic Freeze-Dried PRP to human split-thickness skin graft donor wounds did not generate clinical manifestations of allergic reactions and an increase in IgE.

References

  1. Gonzalez AC, Costa TF, Andrade ZA, Medrado AR. Wound healing - A literature review. An Bras Dermatol. 2016;91(5):614-620. doi:10.1590/abd1806-4841.20164741.
  2. Menke NB, Ward KR, Witten TM, Bonchev DG, Diegelmann RF. Impaired wound healing. Clin Dermatol. 2007;25(1):19-25. doi:10.1016/j.clindermatol.2006.12.005.
  3. Russo V and Watkins J. NHSN Surgical Site Infection Surveillance in 2018. National Center for Emerging and Zoonotic Infectious Disease CDC. 2018. Access from www.cdc.gov/nhsn/pdfs/training/2018/ssi-508.pdf.
  4. Rozman P. Platelet antigens. The role of human platelet alloantigens (HPA) in blood transfusion and transplantation. Transpl Immunol. 2002;10(2-3):165-181. doi:10.1016/s0966-3274(02)00063-1.
  5. Zhang ZY, Huang AW, Fan JJ, et al. The potential use of allogeneic platelet-rich plasma for large bone defect treatment: immunogenicity and defect healing efficacy. Cell Transplant. 2013;22(1):175-187. doi:10.3727/096368912X653183.
  6. Rachmawati T. The effect of Allogenic Freeze Dried Platelet-Rich Plasma in Rabbit Immunological Response. Department of Biology Faculty of Science and Technology. 2015.
  7. Gubina B, Rožman P, Bišcević M, Domanović D, Smrke D. The influence of allogeneic platelet gel on the morphology of human long bones. Coll Antropol. 2014;38(3):865-870.
  8. Jo CH, Shin JS, Lee SY, Shin S. Allogeneic Platelet-Rich Plasma For Rotator Cuff Repair. Acta Ortop Bras. 2017;25(1):38-43. doi:10.1590/1413-785220172501163417.
  9. Croisé B, Paré A, Joly A, Louisy A, Laure B, Goga D. Optimized centrifugation preparation of the platelet rich plasma: Literature review. J Stomatol Oral Maxillofac Surg. 2020;121(2):150-154. doi:10.1016/j.jormas.2019.07.001.
  10. Saputro ID, Ardan AM, Yuniarti WM, and Putra ON. The wound healing effect of allogeneic freeze-dried platelet-rich plasma in a full-thickness wound animal model. Journal of Reports in Pharmaceutical Sciences. 2021;10(1). p71-6. DOI:10.4103/jrptps.JRPTPS_77_19.
  11. Pietrzak WS, Eppley BL. Platelet rich plasma: biology and new technology. J Craniofac Surg. 2005;16(6):1043-1054. doi:10.1097/01.scs.0000186454.07097.bf.
  12. Cole BJ, Karas V, Hussey K, Pilz K, Fortier LA. Hyaluronic Acid Versus Platelet-Rich Plasma: A Prospective, Double-Blind Randomized Controlled Trial Comparing Clinical Outcomes and Effects on Intra-articular Biology for the Treatment of Knee Osteoarthritis [published correction appears in Am J Sports Med. 2017 Apr;45(5):NP10]. Am J Sports Med. 2017;45(2):339-346. doi:10.1177/0363546516665809.
  13. Liao X, Liang JX, Li SH, et al. Allogeneic Platelet-Rich Plasma Therapy as an Effective and Safe Adjuvant Method for Chronic Wounds. J Surg Res. 2020;246:284-291. doi:10.1016/j.jss.2019.09.019.
  14. Camargo Garbin L, McIlwraith CW, Frisbie DD. Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro. BMC Vet Res. 2019;15(1):386. Published 2019 Nov 1. doi:10.1186/s12917-019-2118-z.
  15. Kawase T. Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances. Odontology. 2015;103(2):126-135. doi:10.1007/s10266-015-0209-2.
  16. He M, Guo X, Li T, et al. Comparison of Allogeneic Platelet-rich Plasma With Autologous Platelet-rich Plasma for the Treatment of Diabetic Lower Extremity Ulcers. Cell Transplant. 2020;29:963689720931428. doi:10.1177/0963689720931428.
  17. Kandil MI, Tabl EA, Elhammady AS. Prospective Randomized Evaluation of Local Injection of Allogeneic Growth Factors in Plantar Fasciitis. Foot Ankle Int. 2020;41(11):1335-1341. doi:10.1177/1071100720939066.
  18. Latalski M, Walczyk A, Fatyga M, et al. Allergic reaction to platelet-rich plasma (PRP): Case report. Medicine (Baltimore). 2019;98(10):e14702. doi:10.1097/MD.0000000000014702.
  19. Afify AM, Rashed MM, Mahmoud EA, and El-Beltagi HS. Effect of Gamma Radiation on Protein Profile, Fraction and Solubility's of Three Oil Seeds: Soybean, Peanut, and Sesame. Notulae Botanicae Horti Agrobotanici Clujnapoca. 2011;39(2). p90-98.
  20. Heo SY, Lee HD, and Kim MS. Biomechanical Assessment of Freeze dried cortical bone plate graft defect models. Journal of Veterinary Medicine. 2009;54(4). p183-90. DOI:10.17221/3029-VETMED.
  21. Harder MNC, Arthur V. Gamma Rays’ Effect on Food Allergen Protein [Internet]. New Insights on Gamma Rays. InTech. 2017. DOI:10.5772/67839.
  22. Andia I, Perez-Valle A, Del Amo C, Maffulli N. Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization. Int J Mol Sci. 2020;21(18):6904. Published 2020 Sep 20. doi:10.3390/ijms21186904.
  23. Muraglia A, Ottonello C, Spanò R, et al. Biological activity of a standardized freeze-dried platelet derivative to be used as cell culture medium supplement. Platelets. 2014;25(3):211-220. doi:10.3109/09537104.2013.803529.
  24. Sudrajat TE, Rizaliyana S, and Perdanakusuma DS. Effectiveness of Pre PRP Injection and Post Elevation Flap at Extended Random Flap Rat Skin. Folia Medica Indonesiana. 2020;56(3). p186–191. https://doi.org/10.20473/fmi.v56i3.24545.
  25. Saputro ID, Hutagalung MR, and Wahdini SI. Effect Of Platelet Rich Plasma (PRP) To Autogenous Bone Graft. Folia Medica Indonesiana. 2017;53(1). p18–23. DOI: 10.20473/fmi.v53i1.5485.
  26. Shiga Y, Kubota G, Orita S, et al. Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period. Asian Spine J. 2017;11(3):329-336. doi:10.4184/asj.2017.11.3.329.
  27. Qu W, Wang Z, Hunt C, et al. Platelet-Rich Plasma for Wound Care in the Medicare Population [Internet]. Rockville (MD): Agency for Healthcare Research and Quality (US).2020. Available from: https://www.ncbi.nlm.nih.gov/books/NBK575939/.
  28. Nugraha GL, Septarendra D, and Lesmana T. Comparative study of SOFA, WSESSSS, and CPIRO scoring systems as mortality predictors in a patient with complicated intra-abdominal infection. Bali Medical Journal. 2022;11(3). p1397–1403. DOI:10.15562/bmj.v11i3.3523
  29. Nugraha GL, Danardono E. A retrospective study of factors affecting mortality in patients with complicated intra-abdominal infection. Med J Malaysia. 2022;77(5):612-618.
  30. Asuku M, Yu TC, Yan Q, et al. Split-thickness skin graft donor-site morbidity: A systematic literature review. Burns. 2021;47(7):1525-1546. doi:10.1016/j.burns.2021.02.014.
  31. Rotatori RM, Starr B, Peake M, et al. Prevalence and Risk Factors for Hypertrophic Scarring of Split Thickness Autograft Donor Sites in a Pediatric Burn Population. Burns. 2019;45(5):1066-1074. doi:10.1016/j.burns.2019.02.003.
  32. Karlsson M, Elmasry M, Steinvall I, Sjöberg F, Olofsson P. Scarring at Donor Sites after Split-Thickness Skin Graft: A Prospective, Longitudinal, Randomized Trial. Adv Skin Wound Care. 2020;33(12):1-5. doi:10.1097/01.ASW.0000720256.45983.28.
  33. Legemate CM, Ooms PJ, Trommel N, Middelkoop E, van Baar ME, Goei H, and van der Vlies CH. Patient-reported scar quality of donor-sites following split-skin grafting in burn patients: long-term results of a prospective cohort study. Journal of the International Society for Burn Injuries. 2020;47(2). p315-21. DOI:10.1016/j.burns.2020.12.005.

How to Cite

Bernadetta, Hutagalung, M. R., & Hariani, L. (2023). Clinical manifestations of allergic reactions and immunoglobulin E levels on allogeneic freeze-dried platelet-rich plasma (PRP) application. Bali Medical Journal, 12(1), 1167–1173. https://doi.org/10.15562/bmj.v12i1.4252

HTML
8

Total
2

Share

Search Panel