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Comparison of the histological features in the photoaged animal model

  • Winawati Eka Putri ,
  • Meidyta Sinantryana Widyaswari ,
  • Rizki Amalia ,
  • David Sajid Muhammad ,
  • Nadia Nisaussholihah ,
  • Deny Febriwijaya Romadhani ,


Abstract

Background: Photoaging is a type of aging primarily caused by exposure to radiation, such as Ultraviolet B (UVB). This radiation penetrates the upper epidermis and papillary dermis leading to sunburn, tanning, photoaging, and photocarcinogenesis. Its light is a significant cause of direct deoxyribonucleic acid (DNA) damage. Furthermore, it induces transcription factors, such as NF- and other pro-inflammatory cytokines, the expression of matrix metalloproteinase (MMP), and generates reactive oxygen species (ROS). Therefore, this study aimed to determine the differences in the histologic features of the photoaged animal models.

Methods: There were three groups, including P1, P2, and P3. Group P1 consists of male Wistar rats (Rattus norvegicus), aged 10-12 weeks, with average body weight between 100-150 grams. Group P2 consists of C57BL aged 5-6 weeks with an average weight of 50-75 grams. P3 comprises BABL/c mice aged 5-6 weeks with an average weight of 50-75 grams. They were photoaged for 6 weeks using UV lamps (Ultraviolet B Broadband TL lamps. Phillips TL 20W/01 RS) with a 290-315 nm wavelength.

Results: The results showed a significant difference in epidermal thickness, dermal thickness, and sunburn cell (SBC) between groups after exposure to UVB radiation (p<0.05). Meanwhile, there was no significant difference in their blood vessels (p>0.05).

Conclusion: The exposure to UVB for six weeks affects epidermal and dermal thickness, amount of SBC, and blood vessels in the photoaged animal model.

Keywords: Photoaging epidermal thickness dermal thickness sunburn cell blood vessel

References

  1. Muslim M, Jusuf NK, Putra IB. The dermoscopic features of facial aging among diverse ethnicity in Medan, Indonesia. Bali Med J. 2021;10(2):904–9. Available from: http://dx.doi.org/10.15562/bmj.v10i2.2463
  2. Sawane M, Kajiya K. Ultraviolet light-induced changes of lymphatic and blood vasculature in skin and their molecular mechanisms. Exp Dermatol. 2012;21:22–5. Available from: http://dx.doi.org/10.1111/j.1600-0625.2012.01498.x
  3. Connolly K, Hexsel CL, Lim HW. Photodamage [Internet]. Textbook of Cosmetic Dermatology. Informa Healthcare; 2010. p. 41–6. Available from: http://dx.doi.org/10.3109/9781841847641.004
  4. Tobin DJ. Introduction to skin aging. J Tissue Viability. 2017;26(1):37–46. Available from: http://dx.doi.org/10.1016/j.jtv.2016.03.002
  5. Sayama A, Soushin T, Okada T, Doi K, Nakayama H. Morphological and Biochemical Changes During Aging and Photoaging of the Skin of C57BL/6J Mice. J Toxicol Pathol. 2010/10/05. 2010;23(3):133–9. Available from: https://pubmed.ncbi.nlm.nih.gov/22272024
  6. Slominski AT, Zmijewski MA, Plonka PM, Szaflarski JP, Paus R. How UV Light Touches the Brain and Endocrine System Through Skin, and Why. Endocrinology. 2018;159(5):1992–2007. Available from: https://pubmed.ncbi.nlm.nih.gov/29546369
  7. Bagus Komang Satriyasa, I Gusti Ayu Widianti, I.B.G. Fajar Manuaba. The potential of carrot extract as a sunscreen to prevent apoptosis in white mice (Mus musculus) fibroblast cell cultures exposed to UVB light . Bali Med J. 2022;11(2 SE-ORIGINAL ARTICLE):527–30. Available from: https://www.balimedicaljournal.org/index.php/bmj/article/view/3460
  8. Lestari W, Hajar S, Earlia N, Asrizal CW, Ritonga MA. Effect of arabica Gayo coffee skin in repairing collagen damaged by UVB exposure. Bali Med J. 2022;12(1 SE-ORIGINAL ARTICLE):99–102. Available from: https://www.balimedicaljournal.org/index.php/bmj/article/view/3954
  9. Ahsanuddin S, Lam M, D. Baron E. Skin aging and oxidative stress. AIMS Mol Sci. 2016;3(2):187–95. Available from: http://dx.doi.org/10.3934/molsci.2016.2.187
  10. Sheehan JM, Young AR. The sunburn cell revisited: an update on mechanistic aspects. Photochem & Photobiol Sci. 2002;1(6):365–77. Available from: http://dx.doi.org/10.1039/b108291d
  11. Soetrisno K, Subchan P, Hussana A. The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis. Sains Med. 2021;11(2):89. Available from: http://dx.doi.org/10.30659/sainsmed.v11i2.9094
  12. JOHNSON BE, MANDELL G, DANIELS F. Melanin and Cellular Reactions to Ultraviolet Radiation. Nat New Biol. 1972;235(57):147–9. Available from: http://dx.doi.org/10.1038/newbio235147a0
  13. Kulka M. Mechanisms and Treatment of Photoaging and Photodamage [Internet]. Using Old Solutions to New Problems - Natural Drug Discovery in the 21st Century. InTech; 2013. Available from: http://dx.doi.org/10.5772/56425
  14. Glogau RG. Photo Aging and Aging Skin. In: Rigel D, Weiss R, Linn H, Dover J, editors. Photoaging. 2nd Editio. Canada: Maarced Decker; 2004. p. 65–73.
  15. Mansouri P, Chalangari R, Chalangari KM, Saffarian Z. Skin Aging and Immune System [Internet]. Immunology of Aging. Springer Berlin Heidelberg; 2013. p. 339–68. Available from: http://dx.doi.org/10.1007/978-3-642-39495-9_25
  16. Yaar M, Gilchrest BA. Skin aging. Clin Geriatr Med. 2001;17(4):617–30. Available from: http://dx.doi.org/10.1016/s0749-0690(05)70089-6
  17. CHUNG JH, EUN HC. Angiogenesis in skin aging and photoaging. J Dermatol. 2007;34(9):593–600. Available from: http://dx.doi.org/10.1111/j.1346-8138.2007.00341.x
  18. Shin SW, Jung E, Kim S, Kim J-H, Kim E-G, Lee J, et al. Antagonizing effects and mechanisms of afzelin against UVB-induced cell damage. PLoS One. 2013;8(4):e61971–e61971. Available from: https://pubmed.ncbi.nlm.nih.gov/23626759
  19. Wu P-Y, Huang C-C, Chu Y, Huang Y-H, Lin P, Liu Y-H, et al. Alleviation of Ultraviolet B-Induced Photodamage by Coffea arabica Extract in Human Skin Fibroblasts and Hairless Mouse Skin. Int J Mol Sci. 2017;18(4):782. Available from: https://pubmed.ncbi.nlm.nih.gov/28387707
  20. Kligman LH. The hairless mouse model for photoaging. Clin Dermatol. 1996;14(2):183–95. Available from: http://dx.doi.org/10.1016/0738-081x(95)00154-8
  21. Lesnik RH, Kligman LH, Kligman AM. Agents that cause enlargement of sebaceous glands in hairless mice. I. Topical substances. Arch Dermatol Res. 1992;284(2):100–5. Available from: http://dx.doi.org/10.1007/bf00373378
  22. Sharma MR, Werth B, Werth VP. Animal models of acute photodamage: comparisons of anatomic, cellular and molecular responses in C57BL/6J, SKH1 and Balb/c mice. Photochem Photobiol. 2011/03/09. 2011;87(3):690–8. Available from: https://pubmed.ncbi.nlm.nih.gov/21332482
  23. You Y-J, Wu P-Y, Liu Y-J, Hou C-W, Wu C-S, Wen K-C, et al. Sesamol Inhibited Ultraviolet Radiation-Induced Hyperpigmentation and Damage in C57BL/6 Mouse Skin. Antioxidants (Basel, Switzerland). 2019;8(7):207. Available from: https://pubmed.ncbi.nlm.nih.gov/31284438
  24. Kwak CS, Yang J, Shin C-Y, Chung JH. Topical or oral treatment of peach flower extract attenuates UV-induced epidermal thickening, matrix metalloproteinase-13 expression and pro-inflammatory cytokine production in hairless mice skin. Nutr Res Pract. 2018/01/24. 2018;12(1):29–40. Available from: https://pubmed.ncbi.nlm.nih.gov/29399294
  25. Moloney SJ, Edmonds SH, Giddens LD, Learn DB. THE HAIRLESS MOUSE MODEL OF PHOTOAGING: EVALUATION OF THE RELATIONSHIP BETWEEN DERMAL ELASTIN, COLLAGEN, SKIN THICKNESS AND WRINKLES. Photochem Photobiol. 1992;56(4):505–11. Available from: http://dx.doi.org/10.1111/j.1751-1097.1992.tb02194.x
  26. Moon SE, Youn JI, Kim JA. The effect of ultraviolet-B exposure scheduling on the photodamage of hairless mouse skin. Photodermatol Photoimmunol & Photomed. 2000;16(2):74–7. Available from: http://dx.doi.org/10.1034/j.1600-0781.2000.d01-7.x
  27. Fan Y, Jeong JH, You GY, Park JU, Choi TH, Kim S. An Experimental Model Design for Photoaging. J Craniofac Surg. 2015;26(6):e467–71. Available from: http://dx.doi.org/10.1097/scs.0000000000001902
  28. Hwang E, Park S-Y, Lee HJ, Lee TY, Sun Z, Yi TH. Gallic Acid Regulates Skin Photoaging in UVB-exposed Fibroblast and Hairless Mice. Phyther Res. 2014;28(12):1778–88. Available from: http://dx.doi.org/10.1002/ptr.5198
  29. Choi S-I, Jung T-D, Cho B-Y, Choi S-H, Sim W-S, Han X, et al. Anti‑photoaging effect of fermented agricultural by‑products on ultraviolet B‑irradiated hairless mouse skin. Int J Mol Med. 2019/06/12. 2019;44(2):559–68. Available from: https://pubmed.ncbi.nlm.nih.gov/31198982
  30. Kim HN, Gil CH, Kim YR, Shin HK, Choi BT. Anti-photoaging properties of the phosphodiesterase 3 inhibitor cilostazol in ultraviolet B-irradiated hairless mice. Sci Rep. 2016;6:31169. Available from: https://pubmed.ncbi.nlm.nih.gov/27484958
  31. Bora NS, Mazumder B, Mandal S, Patowary P, Goyary D, Chattopadhyay P, et al. Amelioration of UV radiation-induced photoaging by a combinational sunscreen formulation via aversion of oxidative collagen degradation and promotion of TGF-β-Smad-mediated collagen production. Eur J Pharm Sci. 2019;127:261–75. Available from: http://dx.doi.org/10.1016/j.ejps.2018.11.004
  32. Wibisono EW. Krim Ekstrak Etanol Bawang Putih Tunggal (Allium sativum) Menghambat Penebalan Epidermis Tikus Wistar Jantan (Rattus norvegicus) yang Dipapar Sinar Ultraviolet-B. J Kesehat Andalas. 2020;9(1):1. Available from: http://dx.doi.org/10.25077/jka.v9i1.1275
  33. Suyono H, Sanjaya K, Susanti D. The Role of Antiapoptotic Erythropoietin on Ultraviolet B-Induced Photodamaged Skin Through Inhibition of Sunburn Cells. Folia Medica Indones. 2020;56(2):114. Available from: http://dx.doi.org/10.20473/fmi.v56i2.21229
  34. Toyoda M, Nakamura M, Luo Y, Morohashi M. Ultrastructural characterization of microvasculature in photoaging. J Dermatol Sci. 2001;27:32–41. Available from: http://dx.doi.org/10.1016/s0923-1811(01)00117-7
  35. Jung SK, Lee KW, Byun S, Lee EJ, Kim J-E, Bode AM, et al. Myricetin inhibits UVB-induced angiogenesis by regulating PI-3 kinase in vivo. Carcinogenesis. 2009/12/11. 2010;31(5):911–7. Available from: https://pubmed.ncbi.nlm.nih.gov/20008033
  36. Karthikeyan R, Kanimozhi G, Madahavan NR, Agilan B, Ganesan M, Prasad NR, et al. Alpha-pinene attenuates UVA-induced photoaging through inhibition of matrix metalloproteinases expression in mouse skin. Life Sci. 2019;217:110–8. Available from: http://dx.doi.org/10.1016/j.lfs.2018.12.003

How to Cite

Putri, W. E., Widyaswari, M. S., Amalia, R., Muhammad, D. S., Nisaussholihah, N., & Romadhani, D. F. (2023). Comparison of the histological features in the photoaged animal model. Bali Medical Journal, 12(3), 3064–3069. https://doi.org/10.15562/bmj.v12i3.4410
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Winawati Eka Putri
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Meidyta Sinantryana Widyaswari
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Rizki Amalia
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David Sajid Muhammad
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Nadia Nisaussholihah
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Deny Febriwijaya Romadhani
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