Microbial inhibition test and optimization of temperature, aeration fermentation of endophytic Fusarium sp LBKURCC 41 from Dahlia tuber (Dahlia variabilis)

Dewi Yudiana Shinta; Muhammmad Diki Juliandi; Widyastuti Widyastuti; Herix Sonata MS; Saryono Saryono

doi:10.15562/bmj.v12i3.4879

Microbial inhibition test and optimization of temperature, aeration fermentation of endophytic Fusarium sp LBKURCC 41 from Dahlia tuber (Dahlia variabilis)

Dewi Yudiana Shinta ,

Muhammmad Diki Juliandi ,

Widyastuti Widyastuti ,

Herix Sonata MS ,

Saryono Saryono ,

pdf |
DOI: https://doi.org/10.15562/bmj.v12i3.4879 |
Published: 2023-11-14

Abstract

Link of Video Abstract: https://youtu.be/G8AKREK9E9Q

Introduction: Synthetic drugs can cause toxicity. Finding new natural sources of medicinal materials with low toxicity is one of the efforts to reduce it. This was done by using biotechnology from endophytic fungi growing on dahlia tubers (Dahlia variabilis). This study aimed to find the optimum temperature and aeration in the fermentation of endophytic fungi Fusarium sp LBKURCC 41 in producing secondary metabolites.

Methods: The method of this research was temperature optimization with temperature variations of 10˚C, 27˚C, 37˚C and 40˚C. For aeration, the variation of oxygen provided was 50 ppb and 100 ppb. Both fermentation environment factors were incubated for 5, 10, 15, 20, and 25 days. The activity test was conducted against pathogenic microbes E. Coli, S. aureus, and C. albicans using the agar diffusion method. Positive controls used ketoconazole for Candida albicans and ciprofloxacin for E. coli and Streptococcus aureus bacteria.

Result: The results showed the optimum temperature of the fermentation of endophytic fungi Fusarium sp LBKURCC 41 based on statistical analysis of T-Test for (p < 0.05) is to have the highest inhibition zone diameter value of 10.5 ± 0.62 mm on day 20 against Candida albicans with a positive control of 6.0 ± 0.23 mm. For aeration, the highest inhibition zone value was 12.3 ± 0.12 mm with a positive control of 19.5 ± 0.12 mm.

Conclusion: The optimum temperature of the fermentation is to have the highest inhibition zone diameter value of 10.5 ± 0.62 mm. The ability of endophytic fungi to produce secondary metabolite compounds is determined by the temperature and aeration used.

References

Wali AR, Nursyirwani, Verawaty M, Saryono. Antimicrobial production using endophytic fungus Sporothrix sp. LBKURCC43 by carbon and nitrogen modification. J. Phys.: Conf. Ser. 2019; 1167:012032.
Mutiawati VK. Pemeriksaan mikrobiologi pada Candida albicans. JSK. 2016;16(1):53-63.
Saryono, Hendris S, Fitriyah D, Jose C, Nugroho TT, Ardhi A. Antimicrobial activity and molecular characterization of endophytic fungi strains isolated from Dahlia (Dahlia variabilis). Journal of Chemical and Pharmaceutical Research. 2015;7:201-8.
Saryono, Fitriyah D, Christinejose. Determination of optimum time of antimicrobial production and phytochemical test of crude extract of fermentation of endophytic bacteria Pseudomonas sp from Dahlia plant tubers (Dahlia variabilis). Journal.Ind.Che.Acta. 2013;3(2).
Shinta DY, Yusmarini, Herix Sonata MS, Teruna HY, Saryono. The media variance of production for anti microbe homogeny from the endophyte fungi of dahlia plant seed (Dahlia variabilis). Journal of Chemical and Pharmaceutical Research. 2015;7(9S):239-45.
Shinta DY, Yusmarini, Herix Sonata MS, Teruna HY, Saryono. Optimization of temperature and fermentation media in the production of secondary metabolites by Endophytic Sporothrix sp and it's activity against Candida albicans from Dahlia tubers (Dahlia variabilis). Pakistan Journal of Medical & Health Sciences. 2019;13:1203-7.
Huang WY, Cai YZ, Hyde KD, Corke H, Sun M. Endophytic fungi from Nerium oleander L (Apocynaceae) main constituents and antioxidant activity. World Journal of Microbiology and Biotechnology. 2007;23(9):1253-63.
Gurupavithra S, Jayachitra A. Isolation and identification of endophytic fungi from Ocimum sanctum and analyse its antioxidant properties. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2013;4:1120-5.
Shinta DY, Yusmarini, Herix Sonata MS, Teruna HY, Saryono. Uji bioaktivitas antibakteri senyawa murni dari jamur endofit Sporothrix sp terhadap bakteri Escherichia coli dan Staphylococcus aureus. Dinamika Lingkungan Indonesia. 2019;6(1):37-44.
Martins IM, Cortés JC, Muñoz J, Moreno MB, Ramos M, Clemente-Ramos JA, et al. Differential activities of three families of specific beta(1,3)glucan synthase inhibitors in wild-type and resistant strains of fission yeast. J Biol Chem. 2011;286(5):3484-96.
Pratiwi ST. Mikrobiologi Farmasi. Jakarta: Erlangga; 2008.
Ferdaus F, Wijayanti MO, Retnoningtyas ES, Irawati W. Pengaruh pH, konsentrasi substrat, penambahan kalsium karbonat dan waktu fermentasi terhadap perolehan asam laktat dari kulit pisang, Widya Teknik, 2008;7(1):1-14.

[1] Wali AR, Nursyirwani, Verawaty M, Saryono. Antimicrobial production using endophytic fungus Sporothrix sp. LBKURCC43 by carbon and nitrogen modification. J. Phys.: Conf. Ser. 2019; 1167:012032.

[2] Mutiawati VK. Pemeriksaan mikrobiologi pada Candida albicans. JSK. 2016;16(1):53-63.

[3] Saryono, Hendris S, Fitriyah D, Jose C, Nugroho TT, Ardhi A. Antimicrobial activity and molecular characterization of endophytic fungi strains isolated from Dahlia (Dahlia variabilis). Journal of Chemical and Pharmaceutical Research. 2015;7:201-8.

[4] Saryono, Fitriyah D, Christinejose. Determination of optimum time of antimicrobial production and phytochemical test of crude extract of fermentation of endophytic bacteria Pseudomonas sp from Dahlia plant tubers (Dahlia variabilis). Journal.Ind.Che.Acta. 2013;3(2).

[5] Shinta DY, Yusmarini, Herix Sonata MS, Teruna HY, Saryono. The media variance of production for anti microbe homogeny from the endophyte fungi of dahlia plant seed (Dahlia variabilis). Journal of Chemical and Pharmaceutical Research. 2015;7(9S):239-45.

[6] Shinta DY, Yusmarini, Herix Sonata MS, Teruna HY, Saryono. Optimization of temperature and fermentation media in the production of secondary metabolites by Endophytic Sporothrix sp and it's activity against Candida albicans from Dahlia tubers (Dahlia variabilis). Pakistan Journal of Medical & Health Sciences. 2019;13:1203-7.

[7] Huang WY, Cai YZ, Hyde KD, Corke H, Sun M. Endophytic fungi from Nerium oleander L (Apocynaceae) main constituents and antioxidant activity. World Journal of Microbiology and Biotechnology. 2007;23(9):1253-63.

[8] Gurupavithra S, Jayachitra A. Isolation and identification of endophytic fungi from Ocimum sanctum and analyse its antioxidant properties. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2013;4:1120-5.

[9] Shinta DY, Yusmarini, Herix Sonata MS, Teruna HY, Saryono. Uji bioaktivitas antibakteri senyawa murni dari jamur endofit Sporothrix sp terhadap bakteri Escherichia coli dan Staphylococcus aureus. Dinamika Lingkungan Indonesia. 2019;6(1):37-44.

[10] Martins IM, Cortés JC, Muñoz J, Moreno MB, Ramos M, Clemente-Ramos JA, et al. Differential activities of three families of specific beta(1,3)glucan synthase inhibitors in wild-type and resistant strains of fission yeast. J Biol Chem. 2011;286(5):3484-96.

[11] Pratiwi ST. Mikrobiologi Farmasi. Jakarta: Erlangga; 2008.

[12] Ferdaus F, Wijayanti MO, Retnoningtyas ES, Irawati W. Pengaruh pH, konsentrasi substrat, penambahan kalsium karbonat dan waktu fermentasi terhadap perolehan asam laktat dari kulit pisang, Widya Teknik, 2008;7(1):1-14.

Microbial inhibition test and optimization of temperature, aeration fermentation of endophytic Fusarium sp LBKURCC 41 from Dahlia tuber (Dahlia variabilis)

Abstract

References

How to Cite

Search Panel