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Comparison of five growth factors in the secretomes of hypoxic bone marrow mesenchymal stem cells and hypoxic adipose mesenchymal stem cells

  • Zainurrahman Kurnia Putra ,
  • Ferdiansyah Mahyudin ,
  • Heri Suroto ,

Abstract

Introduction: The Secretome consists of proteins secreted by human cells, containing various serum proteins and growth factors (GFs) that can aid in tissue repair and regeneration. The two most commonly utilized tissues for producing secretomes are Adipose-Derived Mesenchymal Stem Cells (AdMSCs) and Bone Marrow Mesenchymal Stem Cells (BM-MSCs). Hypoxic conditions are also one of the critical preconditioning factors that enhance the efficacy of the secretome. By analyzing and comparing the growth factor profiles between these two cellular sources, we aim to elucidate their potential therapeutic applications and further our understanding of the underlying mechanisms driving their regenerative abilities.

Methods: A randomized controlled post-test-only group design study was conducted at the Cell and Tissue Bank of Dr. Soetomo Hospital. Secretome was derived from AdMSCs and BM-MSCs cultured under hypoxic conditions (5% O2 level). VEGF, PDGF, IGF-1, TGF-β, and bFGF from each group were analyzed by ELISA and compared.

Results: Each group consisted of 7 samples. PDGF levels were significantly higher in the AdMSCs group (7.99 ± 0.644) compared to the BM-MSC group (7.21 ± 0.46); (p = 0.013; p<0.05). Meanwhile, IGF-1 levels were significantly higher in the BM-MSCs group (16.42 ± 1,878) compared to the AdMSCs group (14.2 ± 0.988); (p = 0.021; p<0.05). There were no significant differences in other growth factors between the two groups, including bFGF (0.57 ± 0.05 vs. 0.61 ± 0.07; p = 0.207), VEGF (1,644.00 ± 123.65 vs. 1,588.26 ± 117.80; p = 0.405), and TGF-β (1,323.29 ± 145.36 vs. 1,264.06 ± 121.60; p = 0.425).

Conclusion: No significant differences in VEGF, TGF- β, and bFGF between the two groups. Therefore, AdMSC can be a better alternative to BM-MSC as it is easier to obtain, readily available, and has higher PDGF content.

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How to Cite

Putra, Z. K., Mahyudin, F. ., & Suroto, H. . (2024). Comparison of five growth factors in the secretomes of hypoxic bone marrow mesenchymal stem cells and hypoxic adipose mesenchymal stem cells. Bali Medical Journal, 13(1), 577–581. https://doi.org/10.15562/bmj.v13i1.5155

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Zainurrahman Kurnia Putra
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Ferdiansyah Mahyudin
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Heri Suroto
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